Effluent decontamination by the ibuprofen-mineralizing strain, Sphingopyxis granuli RW412: Metabolic processes

The high global consumption of ibuprofen and its limited elimination by wastewater treatment plants (WWTPs), has led to the contamination aquatic systems this common analgesic metabolites. potentially negative environmental public health effects emerging contaminant have raised concerns, driving demand for technologies. implementation bacteria which mineralize organic contaminants in biopurification used decontaminate water or directly processes WWTPs, is a cheap sustainable means complete before release into environment. In work, an ibuprofen-mineralizing bacterial strain isolated from sediments River Elbe was characterized assayed remediate different ibuprofen-polluted media. Strain RW412, identified as Sphingopyxis granuli, 4.48 Mb genome includes plasmid sequences harbor ipf genes that encode first steps mineralization. Here, we confirm these enzymes initiate CoA ligation ibuprofen, followed aromatic ring activation dioxygenase retroaldol cleavage unequivocally produce 4-isobutylcatechol propionyl-CoA then undergo further degradation. liquid mineral salts medium, eliminated more than 2 mM within 74 h with generation time 16 h. Upon inoculation systems, it repeated doses few days. Furthermore, presence RW412 avoided accumulation ibuprofen-spiked effluent municipal WWTP, removal 7 times faster indigenous microbiota. These results suggest can persist remain active under environmentally relevant conditions, may be useful innovation eliminate urban systems.